Quantitative Analysis of Aconitum Alkaloids

Data acquisition was controlled by Agilent MassHunter Acquisition Software (B.08.00), and MS/MS transitions were performed using Agilent MassHunter Acquisition optimizer software. Data were processed with Quantitative Analysis Software (B.08.00) and Qualitative Analysis Software (B.07.00), respectively. The classification of A. capillaris samples were performed by HCA in the form of the clustering heatmap, which was conducted using with HemI (Heatmap Illustrator, version 1.0, China), a novel software package, to exhibit the differences of PAs with various sample sources [50 (link),51 (link)]. The squared Euclidean distance was used as the metric in the clustering approaches. PCA, a multivariate statistical method to select the principal components representing most of the original variable information [52 (link)], was carried out using OriginPro 2018 SR1 (OriginLab Inc., Northampton, MA, USA).