For propidium iodide (PI) staining (Sigma-Aldrich) skin tissue specimens were fixed with paraformaldehyde and embedded in paraffin [72 (link)]. 5-µm sections were prepared. The slides were analyzed by confocal laser-scanning microscopy. Fluorescent staining was analyzed using a Zeiss LSM 710 CLSM (Zeiss, Jena, Germany) fitted with a Plan-Apochromat 63 × 1.4 objective as previously described [73 (link)]. The PI-stained slides were performed in triplicates.
Riwaldt S., Monici M., Graver Petersen A., Birk Jensen U., Evert K., Pantalone D., Utpatel K., Evert M., Wehland M., Krüger M., Kopp S., Frandsen S., Corydon T., Sahana J., Bauer J., Lützenberg R., Infanger M, & Grimm D. (2017). Preparation of A Spaceflight: Apoptosis Search in Sutured Wound Healing Models. International Journal of Molecular Sciences, 18(12), 2604.
Fluorescent staining analysis using a Zeiss LSM 710 CLSM
control variables
Paraformaldehyde fixation of skin tissue specimens
Paraffin embedding of skin tissue specimens
Preparation of 5-µm sections
controls
The PI-stained slides were performed in triplicates
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