Immunofluorescence Staining of Brain Sections

Immunofluorescence staining was performed according to previous study [17 (link)]. A series of coronal brain sections (10 μm thick) were blocked in 5% bovine serum albumin for 2 h at room temperature and then were incubated with rabbit anti-DRD1 (1:200, Abcam), rabbit anti-IFN-beta receptor (1:50, Santa Cruz), goat anti-ionized calcium-binding adapter molecule 1 (Iba 1, 1:200, Abcam), and rabbit anti-MPO (1:100, Abcam) at 4 °C overnight. After being washed three times with PBS, the sections were incubated with appropriate fluorescence-conjugated secondary antibodies (1:300, Jackson ImmunoResearch) for 2 h at room temperature. The sections were rinsed three times for 5 min each with PBS. The loci around hematoma (black triangle, Fig. 4a) of stained sections were examined with a fluorescence microscope (Leica DMi8). Microphotographs were analyzed with LASX software.