Murine organs were fixed in phosphate-buffered 4% formaldehyde and embedded in paraffin. Three to five micrometres thick sections were stained with hematoxylin and eosin.
Immunofluorescence staining on 5-μm paraffin sections using antibodies against cleaved caspase3 (Cell Signaling, #9664), Goat anti-Rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 (ThermoFisher, #A-11011), and FITC anti-human/mouse Granzyme B Antibody (BioLegend, #515403) was performed as described8 (link),51 (link),52 (link).
Immunofluorescence staining on 5-μm paraffin sections using antibodies against cleaved caspase3 (Cell Signaling, #9664), Goat anti-Rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 (ThermoFisher, #A-11011), and FITC anti-human/mouse Granzyme B Antibody (BioLegend, #515403) was performed as described8 (link),51 (link),52 (link).
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