Immunohistochemical Analysis of Colonic Sections

In situ immunohistochemical analysis of colonic paraffin sections was performed as described previously [13 (link), 17 (link), 18 (link), 23 (link), 24 (link)]. In brief, primary antibodies against cleaved caspase-3 (Asp175, Cell Signaling, USA, 1:200), CD3 (#N1580, Dako, Denmark, dilution 1:10), FOXP3 (FJK-16s, eBioscience, 1:100), B220 (eBioscience, 1:200), and F4/80 (# 14–4801, clone BM8, eBioscience, San Diego, CA, USA, 1:50) were used [13 (link)]. For each animal, the average number of positively stained cells within at least six high power fields (HPF, 400 x magnification) were determined microscopically by a double-blinded investigator [13 (link)].

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Gölz G., Alter T., Bereswill S, & Heimesaat M.M. (2016). The Immunopathogenic Potential of Arcobacter butzleri – Lessons from a Meta-Analysis of Murine Infection Studies. PLoS ONE, 11(7), e0159685.

Publication 2016

Asp175 FJK-16s F4/80

Animal Antibodies Caspase 3 Clone Colonic Dilution Paraffin

Corresponding Organization : Charité - Universitätsmedizin Berlin

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Variable analysis

independent variables

Primary antibodies against cleaved caspase-3 (Asp175, Cell Signaling, USA, 1:200)
Primary antibodies against CD3 (#N1580, Dako, Denmark, dilution 1:10)
Primary antibodies against FOXP3 (FJK-16s, eBioscience, 1:100)
Primary antibodies against B220 (eBioscience, 1:200)
Primary antibodies against F4/80 (# 14–4801, clone BM8, eBioscience, San Diego, CA, USA, 1:50)

dependent variables

Average number of positively stained cells within at least six high power fields (HPF, 400 x magnification)

control variables

Colonic paraffin sections
Immunohistochemical analysis performed as described previously [13, 17, 18, 23, 24]

controls

Positive controls: Not explicitly mentioned
Negative controls: Not explicitly mentioned

Annotations

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