Culture Conditions for Tissue Engineering

The cell culture medium was a mixture of HamF12 and M199 (Sigma-Aldrich, St. Louis, Missouri, USA) (1 : 1), supplemented with 5% FBS (Sigma-Aldrich, St. Louis, Missouri, USA), 1% ascorbic acid (Sigma-Aldrich, St. Louis, Missouri, USA), 0.5% Insulin-Transferrin-Selenium (ITS) (Thermo Fisher Scientific, Waltham, Massachusetts, USA), recombinant human FGF basic (10 ng/mL) (Thermo Fisher Scientific, Waltham, Massachusetts, USA), 10 μM ROCK inhibitor (Y-27632) (Miltenyi Biotec, Bergisch Gladbach, Germany), and 1% PenStrep (Thermo Fisher Scientific, Waltham, Massachusetts, USA) [9 (link)–11 (link)].

Free full text: Click here

Parekh M., Romano V., Ruzza A., Kaye S.B., Ponzin D., Ahmad S, & Ferrari S. (2019). Increasing Donor Endothelial Cell Pool by Culturing Cells from Discarded Pieces of Human Donor Corneas for Regenerative Treatments. Journal of Ophthalmology, 2019, 2525384.

Publication 2019

HamF12 FBS ascorbic acid Insulin-Transferrin-Selenium (ITS) recombinant human FGF basic ROCK inhibitor (Y-27632) PenStrep

Ascorbic acid Cell Cell culture Culture medium Human Insulin Selenium Transferrin Y 27632

Corresponding Organization : Veneto Eye Bank Foundation

Other organizations : University of Liverpool, Fernández-Vega Ophthalmological Institute, Universidad de Oviedo, Royal Liverpool and Broadgreen University Hospital NHS Trust, Moorfields Eye Hospital

Top 5 similar protocols

Variable analysis

independent variables

Mixture of HamF12 and M199 (1:1)
5% FBS
1% ascorbic acid
0.5% Insulin-Transferrin-Selenium (ITS)
Recombinant human FGF basic (10 ng/mL)
10 μM ROCK inhibitor (Y-27632)
1% PenStrep

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

No positive or negative controls were explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!