Immunohistochemical Analysis of Bladder Tumor

The specimens from the bladder patients who underwent surgical resection were cut to 4 μm thick sections, were then formalin-fixed and paraffin-embedded for IHC, as described previously (33 (link)). The primary antibodies used are as follows: CD48 (No.133506, Abcam), MAP4K1 (No.33910, Abcam), IL16 (No.184161, Abcam), CYTIP (No.154847, Abcam), and PTPRC (No.40763, Abcam). Image Pro Plus 6.0 (Media Cybernetics, Bethesda, MD, United States) was employed to measure the positive area of hub genes for quantitative analysis.

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Chen X., Jiang F., Jia C., Liu M., Nan Y., Qu L., Kong Q., Hou F., Luo W., Na W., Jin X, & Tan J. (2019). Comprehensive Gene Expression Analysis in NMIBC Using RNA-seq Reveals New Therapy Strategies. Frontiers in Oncology, 9, 523.

Publication 2019

IL16 Image Pro Plus 6.0

Antibodies Bladder Embedded paraffin Formalin Genes Il16 Map4k1 Patients Ptprc Surgical

Corresponding Organization : Jilin University

Other organizations : First Hospital of Jilin University, Zhengzhou University

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Variable analysis

independent variables

Primary antibodies used: CD48, MAP4K1, IL16, CYTIP, PTPRC

dependent variables

Positive area of hub genes for quantitative analysis

control variables

Tissue specimens were cut to 4 μm thick sections
Tissue specimens were formalin-fixed and paraffin-embedded for IHC
Image Pro Plus 6.0 software was used to measure the positive area of hub genes

controls

No positive or negative controls were explicitly mentioned in the input protocol.

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