Extracting RNA from Tissue and Cells

Total RNA was purified from human femoral head tissue, rat femoral head tissue, and HUVECs using TRIzol (Invitrogen, USA) according to the manufacturer's instructions [26 (link)]. cDNA was generated from the total RNA extracted from tissues or cells with a reverse transcription reaction kit (TAKARA, Japan). The cDNA was used as a template for subsequent qPCR assays. The forward and reverse primers used in this study are shown in Table 1.

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Guo M, & Zhang J. (2022). Vitamin B2 Prevents Glucocorticoid-Caused Damage of Blood Vessels in Osteonecrosis of the Femoral Head. BioMed Research International, 2022, 4006184.

Publication 2022

TRIzol reverse transcription reaction kit

Assays Cdna Cells Femoral head Human Primers Reverse transcription Tissues Trizol

Corresponding Organization : First Affiliated Hospital of Chongqing Medical University

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Variable analysis

independent variables

Tissue type (human femoral head, rat femoral head, HUVECs)

dependent variables

Gene expression levels measured by qPCR

control variables

Total RNA extraction using TRIzol
CDNA generation using reverse transcription kit

controls

No positive or negative controls explicitly mentioned

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