Exome sequencing was done on two platforms, HiSeq 1500 and NextSeq 550 (Illumina, San Diego, CA, USA).
For the NextSeq 550 platform, exome libraries were prepared with the TruSeq DNA Library Preparation Kit (Illumina, San Diego, CA, USA) and the xGen Exome Research Panel (IDT, Integrated DNA Technologies, Coralville, IA, USA) according to the IDT-Illumina TruSeq DNA Exome protocol (Illumina, San Diego, CA, USA). Sequencing was done using NextSeq 550 (Illumina, San Diego, CA, USA) with paired-end sequencing (150 bp) (11 (link), 12 (link)).
For the HiSeq 1500 platform, exome libraries were prepared using the Kapa Library Amplification Kit (Roche, Basel, Switzerland) and NimbleGen SeqCap EZ Exome v3.0 (Roche, Basel, Switzerland). Sequencing was performed on HiSeq 1500 (Illumina, San Diego, CA, USA) with paired-end sequencing (250 bp).
For the NextSeq 550 platform, exome libraries were prepared with the TruSeq DNA Library Preparation Kit (Illumina, San Diego, CA, USA) and the xGen Exome Research Panel (IDT, Integrated DNA Technologies, Coralville, IA, USA) according to the IDT-Illumina TruSeq DNA Exome protocol (Illumina, San Diego, CA, USA). Sequencing was done using NextSeq 550 (Illumina, San Diego, CA, USA) with paired-end sequencing (150 bp) (11 (link), 12 (link)).
For the HiSeq 1500 platform, exome libraries were prepared using the Kapa Library Amplification Kit (Roche, Basel, Switzerland) and NimbleGen SeqCap EZ Exome v3.0 (Roche, Basel, Switzerland). Sequencing was performed on HiSeq 1500 (Illumina, San Diego, CA, USA) with paired-end sequencing (250 bp).
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