Livers were fixed in 4% formalin and then embedded in OCT or paraffin. Neutral lipids were stained with Oil red O [25] (link), and liver collagens were stained with picrosirius red. Hepatic MDA levels were measured using a TBARS assay kit from Cell Biolabs (San Diego, CA. Cat # STA-330). Hepatic hydroxyproline levels were measured using a kit from Cell Biolabs (Cat # STA-675).
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