TEM was utilized
to detect fibril formation at the end of ThT kinetics of fibril formation
as mentioned in previous publication.7 (link),13 (link),14 (link) A volume of 10 μL was applied on a 400-mesh
Formvar carbon-coated copper grid (Electron Microscopy Sciences, Hatfield,
PA). The grids were incubated in contact with α-syn samples
for 1 min. Grids were washed three times with distilled water. They
were carefully air-dried and incubated for 1 min in a fresh solution
of 1% uranyl acetate. After removal of excess stain, acquisition of
pictures was performed with the following settings using a transmission
electron microscope (JEOL 1400 Flash, Japan): accelerating voltage
of 100 kV and magnification of 40k.
to detect fibril formation at the end of ThT kinetics of fibril formation
as mentioned in previous publication.7 (link),13 (link),14 (link) A volume of 10 μL was applied on a 400-mesh
Formvar carbon-coated copper grid (Electron Microscopy Sciences, Hatfield,
PA). The grids were incubated in contact with α-syn samples
for 1 min. Grids were washed three times with distilled water. They
were carefully air-dried and incubated for 1 min in a fresh solution
of 1% uranyl acetate. After removal of excess stain, acquisition of
pictures was performed with the following settings using a transmission
electron microscope (JEOL 1400 Flash, Japan): accelerating voltage
of 100 kV and magnification of 40k.
