Plasma Tau Biomarkers Quantification

Plasma samples were collected according to standard procedures in the clinical routine. Samples were then rapidly frozen for permanent storage at −80°C.
²² (link) Plasma levels of pTau variants pTau‐181 and pTau‐231 were quantified using a custom Single molecule array (Simoa) assay as previously described.
²² (link) pTau‐217⁺ was quantified by Janssen R&D.
²³ (link) Plasma NTA‐tau concentrations were quantified using an in‐house–developed Simoa immunoassay using a Simoa HD‐X platform (Quanterix) at the Clinical Neurochemistry Laboratory (Mölndal, Sweden). Development and validation of the NTA assay has been previously described.
⁹ (link) In brief, plasma NTA assay is comprised by a mouse monoclonal antibody with epitope 6‐18aa (Tau12, BioLegend) used as a detector and a mouse monoclonal antibody with epitope 159‐163aa (HT7, Thermo Scientific) used as the capture antibody.

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Woo M.S., Tissot C., Lantero‐Rodriguez J., Snellman A., Therriault J., Rahmouni N., Macedo A.C., Servaes S., Wang Y., Arias J.F., Hosseini S.A., Chamoun M., Lussier F.Z., Benedet A.L., Ashton N.J., Karikari T.K., Triana‐Baltzer G., Kolb H.C., Stevenson J., Mayer C., Kobayashi E., Massarweh G., Friese M.A., Pascoal T.A., Gauthier S., Zetterberg H., Blennow K, & Rosa‐Neto P. (2023). Plasma pTau‐217 and N‐terminal tau (NTA) enhance sensitivity to identify tau PET positivity in amyloid‐β positive individuals. Alzheimer's & Dementia, 20(2), 1166-1174.

Publication 2023

Simoa HD‐X platform

Corresponding Organization : McGill University

Other organizations : University of Gothenburg, University of Pittsburgh, Janssen (United States), University College London, UK Dementia Research Institute, University of Wisconsin–Madison, National Hospital for Neurology and Neurosurgery, Sahlgrenska University Hospital

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Plasma levels of pTau variants pTau‐181 and pTau‐231
Plasma pTau‐217+ levels
Plasma NTA‐tau concentrations

control variables

Plasma samples were collected according to standard procedures in the clinical routine
Samples were rapidly frozen for permanent storage at −80°C

controls

No positive or negative controls were explicitly mentioned in the input protocol.

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