Two interferents, commercial lipid emulsion (Oliclinomel N-7, 1000 E (20%, Baxter Inc. Lessines, Belgium)) and the native ultralipemic material (NULM) prepared in-house, were used. To prepare NULM, a 100 mL serum pool was collected from approximately 40 residual lipemic serum samples (BD Vacutainer SST II Plus, 5 mL, Becton Dickinson, Franklin Lake, USA) with a triglyceride (TG) concentration > 22.6 mmol/L. The samples from patients who used IVLE or came from the intensive care unit were not included in the study to prevent IVLE contamination. Samples were not frozen, as freeze-thaw cycles would cause errors. They were stored at 2-8 °C for seven days by the stability of the lipids (20 (link)). Then, this pool was centrifuged three times at 45,000xg for 30 minutes in a Hanil Supra 21K (Hanil Scientific Inc., Gimpo, South Korea) refrigerated high-speed centrifuge. After each centrifugation, the supernatant lipid layer was collected carefully. Subsequently, only this lipid-rich portion underwent centrifugation in the following step. At the end of the third collection, we had 5mL of NULM.
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