Human OSCC cell lines (HSC‐2, HSC‐3, and HSC‐4) were obtained from the Japanese Collection of Research Bioresource Cell Bank. HSC‐3pp65 cells transfected with cytomegalovirus (CMV)pp65 antigen and Td‐Tomato in HSC‐3 cells were prepared as previously described.25 (link) The cells were maintained in DMEM (Gibco) supplemented with 10% fetal bovine serum (FBS; HyClone Laboratories, Inc South Logan) and 1% penicillin‐streptomycin (Gibco, Grand Island, NY, USA) in 75 mL flasks at 37°C in 5% CO2 humidified air. Only HSC3pp65 cells were cultured in a culture medium supplemented with 250 μg/mL G418.
Protocol detail
Cultivation of Human OSCC Cell Lines
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Antigen
Cell lines
Cells
Cultured medium
Cytomegalovirus
G418
Human
Japanese
Penicillin
Pp65
Streptomycin
Tomato
Corresponding Organization : Aichi Medical University Hospital
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Variable analysis
independent variables
- HSC-2 cell line
- HSC-3 cell line
- HSC-4 cell line
- HSC-3pp65 cells transfected with cytomegalovirus (CMV)pp65 antigen and Td-Tomato
- Not explicitly mentioned
- DMEM (Gibco) supplemented with 10% fetal bovine serum (FBS; HyClone Laboratories, Inc South Logan) and 1% penicillin-streptomycin (Gibco, Grand Island, NY, USA)
- Culturing conditions: 37°C in 5% CO2 humidified air
- HSC3pp65 cells cultured in medium supplemented with 250 μg/mL G418
- Positive control: Not specified
- Negative control: Not specified
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