Establishing Hyaluronic Acid-Boronic Acid Conjugates

CUR (> 98.0% purity) was obtained from Solarbio (China). 4-Hydroxyphenylboronic acid pinacol ester (PBAP), N,N’-carbonyldiimidazole (CDI), and sodium hyaluronate (HA) were acquired from Macklin (China). The reverse transcription kit was purchased from Accurate Biology (Hunan, China). The RNAeasy™ Animal RNA Extraction Kit and Reactive Oxygen Species Assay Kit were provided by Beyotime (China). The SYBR Prime qPCR Set and Cell Counting Kit-8 (CCK-8) were procured from Bioground (China). Hematoxylin and eosin (H&E) staining kits and tartrate-resistant acid phosphatase staining kits (TRAP Stain Kit) were purchased from Solarbio (China). Cyanine 5.5 (Cy5.5) and lipopolysaccharide (LPS) were sourced from Sigma-Aldrich (USA). Live/Dead BacLight Bacterial Viability Kits were provided by Bestbio (China). All other reagents used were of analytical grade.

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Chen J., Luo A., Xu M., Zhang Y., Wang Z., Yu S., Zhu L., Wu W, & Yang D. (2024). The application of phenylboronic acid pinacol ester functionalized ROS-responsive multifunctional nanoparticles in the treatment of Periodontitis. Journal of Nanobiotechnology, 22, 181.

Publication 2024

RNAeasy™ Animal RNA Extraction Kit Reactive Oxygen Species Assay Kit Hematoxylin and eosin (H&E) staining kits Cyanine 5.5 (Cy5.5) lipopolysaccharide (LPS)

Corresponding Organization :

Other organizations : Stomatological Hospital of Chongqing Medical University, Chongqing Medical University, Chongqing University

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Variable analysis

independent variables

CUR (> 98.0% purity)
4-Hydroxyphenylboronic acid pinacol ester (PBAP)
N,N'-carbonyldiimidazole (CDI)
Sodium hyaluronate (HA)
Cyanine 5.5 (Cy5.5)
Lipopolysaccharide (LPS)

dependent variables

Reactive Oxygen Species
Cell Viability

control variables

All other reagents used were of analytical grade.

controls

Positive control: Not specified
Negative control: Not specified

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