GES-1 (CL-0563) and GC cell line MKN-7 (CL-0574) were purchased from Procell (Wuhan, Hubei, China). GC cells AGS (CRL-1739), SNU-16 (CRL-5974), NCI-N87 (CRL-5822) were from ATCC (Manassas, VA, USA). All cells were cultivated at 37 °C in Roswell Park Memorial Institute-1640 complete medium (PM150110B, Procell) plus 10% FBS with 1% penicillin/streptomycin at 5% CO2.
The overexpressed (oe) DNA plasmids oe-MYH11, oe-TNFRSF14, oe-DNMT3B and control oe-negative control (NC) used for cell transfection were from VectorBuilder (Guangzhou, Guangdong, China). Lipofectamine 2000 (Thermo Fisher) was used for all transfections. Dimethylsulfoxide (DMSO) and DNA methylation inhibitor 5-aZa-CDR (CAS No. 2353-33-5) were purchased from Sigma-Aldrich Chemical Company (St Louis, MO, USA). The GC cells were treated with 5-aZa-CDR dissolved in DMSO at 5 μM for 24 h for DNA demethylation [15 (link)].
The overexpressed (oe) DNA plasmids oe-MYH11, oe-TNFRSF14, oe-DNMT3B and control oe-negative control (NC) used for cell transfection were from VectorBuilder (Guangzhou, Guangdong, China). Lipofectamine 2000 (Thermo Fisher) was used for all transfections. Dimethylsulfoxide (DMSO) and DNA methylation inhibitor 5-aZa-CDR (CAS No. 2353-33-5) were purchased from Sigma-Aldrich Chemical Company (St Louis, MO, USA). The GC cells were treated with 5-aZa-CDR dissolved in DMSO at 5 μM for 24 h for DNA demethylation [15 (link)].
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