Glucose tolerance test (GTT), pyruvate tolerance test, insulin tolerance test (ITT), and triglyceride clearance test were performed as previously described (17 (link)–19 (link)). Liver triglyceride extraction and quantification were performed at the UTSW Metabolic Phenotyping Core using a previously published protocol (17 (link)). Liver glycogen was extracted in water and assayed with a protocol provided in the Glycogen Colorimetric/Fluorometric Assay Kit from BioVision (catalog no. K646-100). Serum parameters (albumin, bilirubin, aspartate transaminase [AST], alanine transaminase [ALT], cholesterol, triglyceride, VLDL, LDL, and direct HDL) were measured and calculated with a VITROS analyzer (Ortho Clinical Diagnostics).
Protocol detail
Metabolic Evaluation of Liver Function
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Alanine transaminase
Albumin
Aspartate transaminase
Assay
Bilirubin
Cholesterol
Colorimetric
Diagnostics
Fluorometric
Glucose tolerance test
Glycogen
Insulin
Liver
Liver glycogen
Pyruvate
Serum
Tolerance
Triglyceride
Corresponding Organization : Eli Lilly (United States)
Other organizations : Chinese Academy of Sciences, Shanghai Institutes for Biological Sciences, Discovery Institute, Sanford Burnham Prebys Medical Discovery Institute
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Protocol cited in 1 other protocol
Variable analysis
independent variables
- Glucose tolerance test (GTT)
- Pyruvate tolerance test
- Insulin tolerance test (ITT)
- Triglyceride clearance test
- Liver triglyceride extraction and quantification
- Liver glycogen extraction and assay
- Serum parameters (albumin, bilirubin, aspartate transaminase [AST], alanine transaminase [ALT], cholesterol, triglyceride, VLDL, LDL, and direct HDL)
- Not explicitly mentioned
- No positive or negative controls were explicitly mentioned in the provided information.
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