Evaluating UGT Inhibition by AB-PINACA
Corresponding Organization : Kyungpook National University
Protocol cited in 3 other protocols
Variable analysis
- AB-PINACA (0.1–100 μM)
- UGT1A1 activity
- UGT1A3 activity
- UGT1A4 activity
- UGT1A6 activity
- UGT1A9 activity
- UGT2B7 activity
- 50 mM Tris buffer (pH 7.4)
- Ultrapooled human liver microsomes (0.2 mg/mL)
- 5 mM UDPGA
- Alamethicin (25 μg/mL)
- 10 mM MgCl2
- Two sets of a cocktail of six UGT probes (set A: 2 μM chenodeoxycholic acid, 0.5 μM SN-38, and 0.5 μM trifluoperazine; set B: 1 μM N-acetylserotonin, 0.2 μM mycophenolic acid, and 1 μM naloxone)
- 60 min incubation at 37 °C in a shaking water bath
- Positive control: The accuracy and relative standard deviation values for six metabolites were 98.0–102.8% and 6.4–9.7%.
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