Ewing sarcoma cell lines were provided by Dr. Timothy Triche Children's Hospital Los Angeles (CHLA, Los Angeles, CA) and the Children's Oncology Group (COG) cell bank (www.cogcell.org ). HuVECs were obtained from Lonza (191027). MRC-5 cells were obtained from ATCC (CCL-171). Human bone marrow-derived mesenchymal stem cell (MSC) lines and hESC-derived neural crest stem cells were obtained as previously described (24 (link)). Identities were confirmed by short tandem repeat profiling. All cell lines were cultured in standard cell culture media, supplemented with 10% FBS (Atlas Biologicals, Inc., F-500-A) at 37°C in 5% CO2. Ewing sarcoma tumor specimens were acquired from the Vanderbilt University pathology archives. Approval from the Vanderbilt University Institutional Review Board was granted prior to tissue acquisition.
Protocol detail
Source and Characterization of Ewing Sarcoma Cell Lines
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Biologicals
Bone marrow
Cell culture
Cell lines
Cells
Cultured media
Ewing sarcoma
F 500
Hesc
Human
Institutional review board
Mesenchymal
Neural crest cells
Short tandem repeat
Stem
Tissue
Tumor
Corresponding Organization : University of Michigan–Ann Arbor
Other organizations : University of Southern California, Vanderbilt University, Van Andel Institute, University of Florida
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Variable analysis
independent variables
- Cell line source (Dr. Timothy Triche Children's Hospital Los Angeles (CHLA, Los Angeles, CA), Children's Oncology Group (COG) cell bank, Lonza, ATCC, Vanderbilt University pathology archives)
- Unspecified
- Cell culture media (standard media, supplemented with 10% FBS)
- Incubation conditions (37°C, 5% CO2)
- Positive control: None specified
- Negative control: None specified
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