Tissue Distribution of Mucetin and Stejnulxin in Mice

Mucetin and stejnulxin were purified as previously reported [9 (link),25 (link),57 (link)]. BALB/c mice (6-week-old) of either sex were anesthetized under anesthesia respirator (R540IP, RWD Life Science) as the manufacturer’s instructions, and perfused with normal saline 30 min after tail vein injection of mucetin and stejnulxin. Liver, lung and kidney were fixed with 4% formalin, dehydrated by 40% sucrose solution and then cut into 10-µm-thick sections on a freezing microtome (CryoStar NX50 OP, Thermofisher, Waltham, MA, USA). The sections were subsequently stained with hematoxylin and eosin for further analysis as previously reported [62 (link)]. Animal protocol in this work, SMKX2017026, was reviewed and approved by the Animal Care and Use Committee at Kunming Institute of Zoology, Chinese Academy of Sciences in December 2017.