Four milliliters of venous blood (2 ml preoperative and 2 ml postoperative) were collected into an EDTA test tube from each study participant by Medical Laboratory Science professionals. Hemoglobin concentration was determined using UniCel DxH 800 (Beckman Coulter, USA) automated hematology analyzer by spectrophotometry method15 (link). Hemoglobin concentration was analyzed at the hospital hematology laboratory and recorded for each study participant using a laboratory result registration form.