Nanoparticle-based Cancer Diagnostics and Therapy

Cetyl trimethylammonium bromide (CTAB), tetraethyl orthosilicate (TEOS), sodium hydroxide (NaOH), ethyl acetate, 3-(Trimethoxysilyl)-1-propanethiol, ammonium nitrate (NH₄NO₃), dimethyl sulfoxide (DMSO), 1,2-dibromoethane, n-hexane, benzyl ether, iron (Ⅲ) acetylacetonate, and oleylamine were obtained from Sigma Aldrich (Dorset, UK). 2′,7′-dichlorodihyofluorescin diacetate (DCFH-DA), and MTT were purchased from Sigma Aldrich (Saint Louis, MO, USA). Fluorescein isothiocyanate (FITC)-labeled annexin-V/propidium iodide (PI) was obtained from ApoFlowEx FITC kit, EXBIO, (Prague, Czech Republic). TRIzol reagent was purchased from Invitrogen (Carlsbad, CA, USA). SYBR green master mix was obtained from Biofact (Daejeon, Republic of Korea). Human ovarian cancer OVCAR-3 and human breast cancer MDA-MB-231 cell lines were obtained from the National Cell Bank of Iran, Pasteur Institute (Tehran, Iran). Amine-modified MUC-1 aptamer with the sequence of (5′-NH₂ (C6) GCAGTTGATCCTTTGGATACCCTGG-3′) [71 (link)] was purchased from Bioneer (Daejeon, Republic of Korea). All other media and cell culture components not listed were purchased from SPL life sciences (Geumgang, Republic of Korea) and Gibco (Paisley, UK).