All in vivo and in vitro experiments were carried out with the S. aureus strain USA300. S. aureus was cultivated in brain heart infusion (BHI) medium (Thermo Fisher Scientific, Waltham, MA, US) at 37°C while shaking at 150 rpm. The bacteria were harvested during the mid-logarithmic phase and adjusted to the appropriate optical density (OD) at 600nm for the respective experiments in phosphate-buffered saline (PBS). The bacteria were then either used freshly for in vivo infection of mice or stored at -80 °C until further usage in in vitro experiments.
For heat-killing, the adjusted bacteria stock was incubated at 95°C for 5 minutes while shaking at 500 rpm. A fraction of the heat-killed bacteria was plated on Müller-Hinton (MH) plates. Plates were incubated for 5 days at 37°C to confirm heat-killing of the bacteria.
For heat-killing, the adjusted bacteria stock was incubated at 95°C for 5 minutes while shaking at 500 rpm. A fraction of the heat-killed bacteria was plated on Müller-Hinton (MH) plates. Plates were incubated for 5 days at 37°C to confirm heat-killing of the bacteria.
