Huh-7 cell line (3111C0001CCC000679) was obtained from China Center for Type Culture Collection (CCTCC; Beijing, China) and Dulbecco’s Modified Eagle’s Medium (DME-H-21 4.5 g/Liter Glucose; Invitrogen) supplemented with 10% FBS (Invitrogen). HCCLM3 (C6303) was obtained from Beyotime (Shanghai, China) and cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% FBS (Invitrogen).
The human liver cancer cell line Huh-7 and HCCLM3 were exposed to gradually increased concentrations of recombinant human TRAIL (rhTRAIL; 1, 10, 100, and 1,000 ng/ml) as described previously (26 (link), 33 (link), 34 (link)) for establishing the TRAIL-resistant Huh-7 (R) and HCCLM3 (R).
The human liver cancer cell line Huh-7 and HCCLM3 were exposed to gradually increased concentrations of recombinant human TRAIL (rhTRAIL; 1, 10, 100, and 1,000 ng/ml) as described previously (26 (link), 33 (link), 34 (link)) for establishing the TRAIL-resistant Huh-7 (R) and HCCLM3 (R).
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