Adult Male Sprague-Dawley rats (Experimental Animal Center of Xi’an Jiaotong University, Xi’an, China) weighing 270–320 g were used in this study (n = 130 rats). Room temperature was kept constant at 22 ± 2°C and the light/dark cycle was 12 h/12 h. Rats had free access to water and food. All experimental procedures were conducted according to the National Institute of Health Guide for the Care and Use of Laboratory Animals (NIH Publications No. 8,023, revised 1978), and approved by the Ethics Committee for Animal Experimentation of Xi’an Jiaotong University. All efforts were made to minimize the number of animals used and their suffering.
Desipramine hydrochloride, 6-OHDA hydrochloride, and apomorphine hydrochloride were purchased from Sigma–Aldrich (Sigma–Aldrich, St. Louis, MO, USA). 6-OHDA was dissolved in artificial cerebrospinal fluid containing 0.02% ascorbic acid; apomorphine was prepared in saline with 0.02% ascorbic acid; desipramine was dissolved in saline. These drugs were prepared on the day of use.
Desipramine hydrochloride, 6-OHDA hydrochloride, and apomorphine hydrochloride were purchased from Sigma–Aldrich (Sigma–Aldrich, St. Louis, MO, USA). 6-OHDA was dissolved in artificial cerebrospinal fluid containing 0.02% ascorbic acid; apomorphine was prepared in saline with 0.02% ascorbic acid; desipramine was dissolved in saline. These drugs were prepared on the day of use.
Free full text: Click here
