Bacterial Reference Strain Cultivation and DNA Extraction

Reference strains of S. agalactiae, Streptococcus uberis, Streptococcus dysgalactiae, Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Pasteurella multocida, Salmonella, Proteus mirabilis, Bacillus cereus, Serratia marcescens, and Shigella sonnei were kept in our laboratory. All strains were grown at 37°C at 150 revolutions per minute (rpm) for 12 h. Genomic DNA was extracted with a D3350 bacterial DNA kit purchased from Omega.

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Zhu L., Gong F., Liu X., Sun X., Yu Y., Shu J, & Pan Z. (2023). Integrating filter paper extraction, isothermal amplification, and lateral flow dipstick methods to detect Streptococcus agalactiae in milk within 15 min. Frontiers in Veterinary Science, 10, 1100246.

Publication 2023

Bacillus cereus Bacterial dna Escherichia coli Genomic Klebsiella pneumoniae Pasteurella multocida Proteus mirabilis Salmonella Serratia marcescens Shigella sonnei Staphylococcus aureus Strains Streptococcus dysgalactiae Streptococcus uberis

Corresponding Organization : Nanjing Agricultural University

Other organizations : China Animal Health and Epidemiology Center

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Variable analysis

independent variables

Reference strains of S. agalactiae, Streptococcus uberis, Streptococcus dysgalactiae, Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Pasteurella multocida, Salmonella, Proteus mirabilis, Bacillus cereus, Serratia marcescens, and Shigella sonnei

dependent variables

Not explicitly mentioned

control variables

Growth at 37°C
Growth at 150 revolutions per minute (rpm)
Growth for 12 h

controls

No positive or negative controls were explicitly mentioned

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