Tumor Cell Dissociation and Flow Cytometry

Freshly harvested tumors were dissociated to single cell suspensions and stained with fluorochrome-conjugated immune phenotyping antibodies for lymphocyte and myeloid populations for flow cytometry purposes as previously described17 (link) and detailed in online supplemental materials. Fully stained samples were run through a BD LSRFortessa X-20 Cell Analyzer using BD FACSDiva software (BD Biosciences). Post-acquisition analyses were performed using Omiq.ai (https://app.omiq.ai/) following the recommended pipeline (see also online supplemental materials).

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Perez-Lanzon M., Carbonnier V., Cordier P., De Palma F.D., Petrazzuolo A., Klein C., Arbaretaz F., Mangane K., Stoll G., Martins I., Fohrer Ting H., Paillet J., Mouillet-Richard S., Le Corre D., Xiao W., Sroussi M., Desdouets C., Laurent-Puig P., Pol J., Lopez-Otin C., Maiuri M.C, & Kroemer G. (2023). New hormone receptor-positive breast cancer mouse cell line mimicking the immune microenvironment of anti-PD-1 resistant mammary carcinoma. Journal for Immunotherapy of Cancer, 11(6), e007117.

Publication 2023

BD LSRFortessa X-20 Cell Analyzer BD FACSDiva software

Antibodies Cell Flow cytometry Fluorochrome Lymphocyte Populations Tumors

Corresponding Organization :

Other organizations : Institut Gustave Roussy, Immunité et Cancer, Inserm, Sorbonne Université, Université Paris Cité, Centre de Recherche des Cordeliers, Sorbonne Paris Cité, Institut Universitaire de France, Hôpital Européen, Hôpital Européen Georges-Pompidou, Universidad de Oviedo

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Variable analysis

independent variables

Tumor dissociation into single cell suspensions

dependent variables

Lymphocyte and myeloid population profiles measured by flow cytometry

control variables

Fluorochrome-conjugated immune phenotyping antibodies used for staining
BD LSRFortessa X-20 Cell Analyzer used for sample analysis
BD FACSDiva software used for data acquisition
Omiq.ai used for post-acquisition data analysis

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