Pharmacokinetics of SZ-A in Rats

Twenty-four SD rats were fasted for 12 h (free access to water) and randomly classified into three groups (n = 8; half male and female) that received different doses of SZ-A solution (25, 50, and 200 mg/kg) via oral administration. After completion of dose administration, blood samples (0.3 mL) were collected through an indwelling catheter from the jugular vein at predetermined time points (5, 15, 30, and 45 min, 1, 2, 4, 8, and 24 h). The blood was centrifuged at 5,000 rpm for 10 min to separate the plasma. All plasma samples were stored frozen at −80°C until liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) analysis (AB SCIEX Triple Quad™ 4,500 mass spectrometer, Applied Biosystems Inc., United States).