Structural Characterization of Chryseochelin

UHPLC–HR–MS was applied to determine accurate masses of siderophores and to predict their molecular formula. MS/MS fragmentation spectra were collected with the conditions described earlier. NMR data of purified chryseochelin A were measured in D₂O on a Bruker AV-600 MHz instrument equipped with a TCI CryoProbe. Thereby, the following experiments were included: ¹H, ¹³C, distortionless enhancement by polarization transfer (DEPT) 90 and 135, heteronuclear single quantum correlation spectroscopy (HSQC), correlation spectroscopy (COSY), heteronuclear multiple bond correlation (HMBC), total correlation spectroscopy (TOCSY), and nuclear overhauser and exchange spectroscopy (NOESY). Ultraviolet-visible (UV/VIS) spectra of chryseochelin A, its ferric complex and photoproduct were recorded on a NanoDrop 2000c spectrophotometer (Thermo Scientific).

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Rehm K., Vollenweider V., Gu S., Friman V.P., Kümmerli R., Wei Z, & Bigler L. (2023). Chryseochelins—structural characterization of novel citrate-based siderophores produced by plant protecting Chryseobacterium spp.. Metallomics: Integrated Biometal Science, 15(3), mfad008.

Publication 2023

Ms ms Siderophores Spectroscopy

Corresponding Organization : Nanjing Agricultural University

Other organizations : Peking University, Center for Life Sciences, University of York

Top 5 similar protocols

Variable analysis

independent variables

UHPLC–HR–MS applied to determine accurate masses of siderophores
MS/MS fragmentation spectra collected with the conditions described earlier
NMR data of purified chryseochelin A measured in D2O on a Bruker AV-600 MHz instrument equipped with a TCI CryoProbe

dependent variables

Accurate masses of siderophores
MS/MS fragmentation spectra
NMR data of purified chryseochelin A

control variables

Distortionless enhancement by polarization transfer (DEPT) 90 and 135
Heteronuclear single quantum correlation spectroscopy (HSQC)
Correlation spectroscopy (COSY)
Heteronuclear multiple bond correlation (HMBC)
Total correlation spectroscopy (TOCSY)
Nuclear overhauser and exchange spectroscopy (NOESY)

controls

Positive control: Ultraviolet-visible (UV/VIS) spectra of chryseochelin A and its ferric complex
Negative control: Ultraviolet-visible (UV/VIS) spectra of chryseochelin A photoproduct

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