Integrated Molecular Profiling for Neuropathologic Diagnostics

Neuropathologic diagnostics was performed in concordance with the WHO classification from 2016 [9 (link)]. The following molecular data were obtained as part of daily routine: IDH mutation status was obtained by immunohistochemistry or direct sequencing of the mutation hotspot region for all patients [16 (link)]. 1p/19q codeletion status was available for all patients. Genome wide methylation analysis with copy number analysis was available for 171 patients (83%) and was generated using the Illumina HumanMethylation450 (450 k) or Methylation EPIC (850 k) array platforms as described [17 (link)].

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Dao Trong P., Gluszak M., Reuss D., von Deimling A., Wick A., König L., Debus J., Herold-Mende C., Unterberg A, & Jungk C. (2023). Isocitrate-dehydrogenase-mutant lower grade glioma in elderly patients: treatment and outcome in a molecularly characterized contemporary cohort. Journal of Neuro-Oncology, 161(3), 605-615.

Publication 2023

HumanMethylation450 (450 k) EPIC (850 k)

Diagnostics Genome Immunohistochemistry Methylation Mutation Patients

Corresponding Organization : University Hospital Heidelberg

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Variable analysis

independent variables

IDH mutation status
1p/19q codeletion status

dependent variables

Neuropathologic diagnostics in concordance with the WHO classification from 2016
Genome wide methylation analysis with copy number analysis

control variables

Immunohistochemistry or direct sequencing of the mutation hotspot region for IDH mutation status
Illumina HumanMethylation450 (450 k) or Methylation EPIC (850 k) array platforms for genome wide methylation analysis with copy number analysis

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