Tilapia Collagen Sponge Characterization

The collagen of tilapia was extracted by the method of acidase combination, and after moderate freeze-drying, it was prepared into collagen concentrate, placed in a mold, and then freeze-dried to obtain collagen sponge with certain spatial structure. The collagen sponge was prepared into a 0.3✕0.5✕0.5 mm³ cube, sprayed with gold (7Pa, 15mA, 200s), and its spatial structure, porosity, pore size, etc. were observed under a scanning electron microscope to determine whether its pore structure was suitable for cell adhesion and crawling.

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Wang L., Qu Y., Li W., Wang K, & Qin S. (2023). Effects and metabolism of fish collagen sponge in repairing acute wounds of rat skin. Frontiers in Bioengineering and Biotechnology, 11, 1087139.

Publication 2023

Cell adhesion Collagen Freeze Gold Mold Scanning electron microscope Sponge Tilapia

Corresponding Organization : Chinese Academy of Sciences

Other organizations : University of Chinese Academy of Sciences

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Variable analysis

independent variables

Method of acidase combination
Freeze-drying of collagen concentrate
Spraying of gold (7Pa, 15mA, 200s)

dependent variables

Spatial structure of collagen sponge
Porosity of collagen sponge
Pore size of collagen sponge

control variables

Molding and freeze-drying of collagen concentrate to obtain collagen sponge
Preparation of collagen sponge into a 0.3x0.5x0.5 mm^3 cube

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