To extract total protein, both HepG2 and Huh7 cells were lysed with RIPA Lysis Buffer (Beyotime Institute of Biotechnology) as described previously (25 (link)). The lysate was centrifuged at 1,200 × g at 4°C for 10 min. The protein amount in the supernatant was quantified using a BCA assay. Subsequently, protein specimens (40 µg/lane) were added onto SDS-PAGE, and electrophoresis at 60 V. Proteins were then transferred to a polyvinylidene difluoride membrane (EMD Millipore). After blocking with 5% (w/v) non-fat dry milk at 37°C for 1 h, the membranes were incubated with primary antibodies against β-actin (1:2,000 dilution; cat. no. ab8226) and hnRNPA2B1 (1:1,000 dilution; cat. no. ab31645) at 4°C overnight. All antibodies were purchased from Abcam. Next, HRP-conjugated secondary antibodies (1:5,000 dilution; cat. no. HRP-60008, ProteinTech Group, Inc.) were added and incubated for 1 h at room temperature. The protein bands were detected using chemiluminescence (Pierce; Thermo Fisher Scientific, Inc.) and visualized on a Tanon 5200 Imaging system (Tanon Science & Technology Co., Ltd.).