HEK293 human kidney
cells were cultured in Dulbecco’s modified Eagle medium (DMEM)
and high-glucose GlutaMax culture medium supplemented with 10% fetal
bovine serum (FBS) at 37 °C, 5% CO2. The cells (0.5
× 106) were seeded in each well of 48-well plates
(Thermo Fisher Scientific Nunc coated with poly-d -lysine
in-house) 24 h prior to transduction with the baculoviruses. To stimulate
the expression of proteins, sodium butyrate was added with the viruses
at a final concentration of 5 mM (as per in-house optimization).
cells were cultured in Dulbecco’s modified Eagle medium (DMEM)
and high-glucose GlutaMax culture medium supplemented with 10% fetal
bovine serum (FBS) at 37 °C, 5% CO2. The cells (0.5
× 106) were seeded in each well of 48-well plates
(Thermo Fisher Scientific Nunc coated with poly-
in-house) 24 h prior to transduction with the baculoviruses. To stimulate
the expression of proteins, sodium butyrate was added with the viruses
at a final concentration of 5 mM (as per in-house optimization).
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