The assay was performed according to the Italian Environmental
Agency guidelines.29 Briefly, seeds of L. sativum and S. lycopersicum not treated with fungicides were preliminary checked for vitality
in distilled water in the dark at 25 ± 1 °C (germination
rates >90%). Crude extracts were tested at six doses (100, 30,
15,
7.5, 3.75, and 1.8% v/v), and distilled water was used as negative
control. Three replicates per treatment were arranged by wetting a
Whatman no. 1 filter paper with 2 mL of each solution. Ten seeds for
each replicate were distributed on the filter and incubated at 25
± 1 °C in the dark for 72 h (L. sativum) or 144 h (S. lycopersicum). At the
end of the incubation time, complete sprouts (≥1 mm) and root
lengths were evaluated to calculate the germination index (GI) using
the eq. , where St and Sc are the mean number of germinated seeds in
treatments and control samples, respectively, and Lt and Lc are the mean root
length of treatments and control samples, respectively. Results were
expressed as GI ± standard deviation (SD). The statistical correlation
between groups was analyzed by Student’s t-test.
Agency guidelines.29 Briefly, seeds of L. sativum and S. lycopersicum not treated with fungicides were preliminary checked for vitality
in distilled water in the dark at 25 ± 1 °C (germination
rates >90%). Crude extracts were tested at six doses (100, 30,
15,
7.5, 3.75, and 1.8% v/v), and distilled water was used as negative
control. Three replicates per treatment were arranged by wetting a
Whatman no. 1 filter paper with 2 mL of each solution. Ten seeds for
each replicate were distributed on the filter and incubated at 25
± 1 °C in the dark for 72 h (L. sativum) or 144 h (S. lycopersicum). At the
end of the incubation time, complete sprouts (≥1 mm) and root
lengths were evaluated to calculate the germination index (GI) using
the eq. , where St and Sc are the mean number of germinated seeds in
treatments and control samples, respectively, and Lt and Lc are the mean root
length of treatments and control samples, respectively. Results were
expressed as GI ± standard deviation (SD). The statistical correlation
between groups was analyzed by Student’s t-test.
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