Immunoblotting for Cell Signaling Proteins

Immunoblotting experiments were conducted as described before (23 (link)). Primary antibodies to LATS1, LATS2, cleaved caspase-3, caspase-3 and p53 were obtained from Cell Signaling, USA. β-actin (Sigma-Aldrich, USA) was used as a loading control. The band intensity of each target was quantified with Image J software.

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Luo S.Y., Kwok H.H., Yang P.C., Ip M.S., Minna J.D, & Lam D.C. (2020). Expression of large tumour suppressor (LATS) kinases modulates chemotherapy response in advanced non-small cell lung cancer. Translational Lung Cancer Research, 9(2), 294-305.

Publication 2020

LATS1 LATS2 cleaved caspase-3 caspase-3 β-actin

Actin Antibodies Caspase 3 Lats1 Lats2

Corresponding Organization : University of Hong Kong

Other organizations : National Taiwan University Hospital, The University of Texas Southwestern Medical Center

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Variable analysis

independent variables

Primary antibodies to LATS1, LATS2, cleaved caspase-3, caspase-3, and p53

dependent variables

Band intensity of each target

control variables

β-actin (Sigma-Aldrich, USA) used as a loading control

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