Colonic Tumor Induction in HFD Mice

C57BL/6J mice (6 weeks old) were housed at Tulane University School of Medicine according to the guidelines of the Tulane Institutional Animal Care and Use Committee (protocol number 1161). One group of mice was maintained on a standard chow diet (RD), and the other on a high-fat chow diet (HFD) (60% kcal/fat) (D12492, Research Diets, New Brunswick, NJ). In addition, colonic tumors were induced in experimental mice by a single azoxymethane (AOM, Sigma, St. Louis, MO, USA) intraperitoneal injection of 10 mg/kg, followed by three separate 5-day cycles of 2.5% dextran sulfate sodium (DSS, MP Biomedicals, San Diego, CA, USA) added to drinking water, as we described before [8 (link)]. Transcriptomic analysis was performed after RNA-seq from the colons of these mice (n = 3 for each group) and is available through NCBI’s Sequence Read Archive (SRP093363) [8 (link)].