Commercial rice flour (Glutal, Santa Fe, Argentina), cassava starch (Dimax, Córdoba, Argentina) and full-fat active soy flour (NICCO, Córdoba, Argentina), compressed yeast (Calsa, Buenos Aires, Argentina), shortening (Dánica, Buenos Aires, Argentina) and salt (Dos Anclas, Buenos Aires, Argentina) were used for gluten-free bread formulation.
Gleditsia triacanthos pods were manually collected from trees in Córdoba (center and northwest region of the province), Argentina. The seeds were mechanically separated from the pods and manually cleaned and classified. The seeds were milled in a hammer grinder (Pulverisette 16, Fritsch, Idar-Oberstein, Germany) [26 (link)]. Additionally, commercial Guar and Xanthan gums (Saporiti, Buenos Aires, Argentina) were used as controls to compare the galactomannans fraction extracted from Gt seeds in gluten-free bread formulation, considering that both gums are among the most popular hydrocolloids used in these products.
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