Psoriasis Induction and Histological Evaluation in BALB/c Mice

BALB/c mice were utilised in the in vivo studies. Test animals were kept at a temperature condition of 25 ± 2 °C, with relative humidity of 45% ± 5% for 12 h of dark and light cycle with no curtailing of food and drink. BALB/c mice were distributed into 6 groups, each with 5 animals. Hair was removed from the dorsal surface of the mice using Veet hair removal cream, and psoriasis was induced by applying Imiquimod cream 5% daily [49 (link),50 (link)] (12.5 mg) on each mouse’s shaved left ear and back for eight days. Except for the control normal mice group, details can be found in Table 2. The clinical Psoriasis Area and Severity Index (PASI) was identified as the best grading system based on the severity of inflammation. After eight days of treatment, animals were subject to cervical dislocation, and skin and spleen were removed and kept in a 10% formalin solution. Haematoxylin/eosin dye was used for staining the samples. These samples were compared to a control skin sample under a light microscope (Motic digital microscope, DMB series) [51 (link)].