Antibody Sources and Expression Vectors

Anisomycin, the polyclonal rabbit anti-B4GALNT2 antibody (HPA015721), the monoclonal mouse anti-GFP (G1546), the monoclonal mouse anti-Flag M2 (F3165), the polyclonal rabbit anti-TMEM165, and the mouse anti-β-actin antibodies were purchased from Sigma-Aldrich (Saint Quentin Fallavier, France). Anti-Sd^a (KM694) antibody was a kind gift from Dr. Taeko Dohi and Dr. Akiko Furuya (Biologics Research Laboratories, Research Division, Kyowa Hakko Kirin Co., Tokyo, Japan). The polyclonal goat anti-mouse horseradish peroxidase-conjugated IgG was purchased from Agilent Technologies (Santa Clara, CA, USA). The polyclonal goat anti-mouse horseradish peroxidase-conjugated IgM was purchased from Fisher Scientific (Illkirch, France). The polyclonal goat anti-rabbit horseradish peroxidase-conjugated IgG was purchased from Sigma-Aldrich (Saint Quentin Fallavier, France). The secondary antibodies Alexa fluor^® 488 anti-rabbit and Alexa fluor^® 568 anti-rabbit were from Life Technologies (Fisher Scientific, Illkirch Graffenstaden, France). The polyclonal rabbit anti-Calnexin was purchased from Enzo life technologies (Villeurbanne, France). The GFP expression vector pFx was a kind gift of Dr. Jack Röhrer (University of Zurich), the expression vectors pEGFP-N1 and pmCherry-N1 were from Clontech, whereas p3×FLAG-CMVTM-10 was from Sigma.