Isolation of Lasiodiplodia theobromae from Mango

The pure culture of L. theobromae was isolated from mango fruit cultivar “Nam Dok Mai Si Thong” following the method of Khan et al. [36 (link)]. Briefly, a small part of decaying mango fruit tissue near the stem end was cut using a sterile blade and placed on the potato dextrose agar (PDA) contained in Petri dishes. The Petri dishes were sealed with Parafilm and incubated in an ambient condition (30 ± 2 °C) until mycelial growth was observed. Subculturing was performed up to 3 times to isolate the pure culture of L. theobromae. Pure culture was verified using a light microscope and based on the colony and conidial characteristics.