According to the expression of cell surface markers in CD4+ T cells, they could be classified as: Th1 cells (CXCR3+/CCR6-), Th17 cells (CXCR3-/CCR6+) and Th2 cells (CXCR3-/CCR6-). Regarding the activation status, each one could be divided into quiescent cells (ICOS-/PD1-), early activated cells (ICOS+/PD-1-), late activated cells (ICOS+/PD-1+) and exhausted or senescent cells (ICOS-/PD-1+) [32 (link)].
Characterizing CD4+ T Cell Subsets
According to the expression of cell surface markers in CD4+ T cells, they could be classified as: Th1 cells (CXCR3+/CCR6-), Th17 cells (CXCR3-/CCR6+) and Th2 cells (CXCR3-/CCR6-). Regarding the activation status, each one could be divided into quiescent cells (ICOS-/PD1-), early activated cells (ICOS+/PD-1-), late activated cells (ICOS+/PD-1+) and exhausted or senescent cells (ICOS-/PD-1+) [32 (link)].
Corresponding Organization : Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública
Other organizations : Universidad Complutense de Madrid
Variable analysis
- Anti-CCR6-PB
- Anti-PD1-PCy7
- Anti-CD3-PerCP5.5
- Anti-CXCR3-PE
- Anti-ICOS-APCH7
- Anti-CD4-KO
- Proportions of Th subsets (Th1, Th17, Th2)
- EDTA-treated whole blood
- No positive or negative controls were explicitly mentioned.
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