1H-NMR spectra were recorded on a Hermes-Varian Mercury Plus 300 (300 MHz) spectrometer at room temperature. Chemical shifts were recorded in parts per million (ppm) using residual solvent peaks as internal references [MeOH-d4 δ: 4.5 (1H) and DMSO-d6 δ: 2.5]. Electro spray ionization mass spectra for the synthesized ligand molecules were obtained using a Waters (Micromass) LCT® mass spectrometer (Waters, Beverly, MA) similarly as described previously.46 (link) High-resolution Fourier transformed ion cyclotron resonance (FT-ICR) mass spectra before and after UV irradiation were obtained on a 7 T LTQ/FT (Thermo Scientific, Waltham, MA), similarly as described in our previous publication.11 (link) DLS was carried out using a Malvern Zetasizer Nano. All DLS measurements of individual samples were performed three times after 120 sec equilibration.
Protocol detail
NMR, Mass Spectrometry, and DLS Analysis
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Other organizations : University of Wisconsin–Madison
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Variable analysis
independent variables
- Magnetic field strength (300 MHz)
- Solvent used for NMR (MeOH-d4 and DMSO-d6)
- Ionization method for mass spectrometry (Electro spray ionization)
- UV irradiation before and after FT-ICR mass spectrometry
- Chemical shifts (in ppm) in NMR spectra
- Mass spectra of synthesized ligand molecules
- High-resolution FT-ICR mass spectra before and after UV irradiation
- Dynamic light scattering (DLS) measurements
- Temperature (room temperature)
- Equilibration time for DLS measurements (120 sec)
- Positive control: Not specified
- Negative control: Not specified
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