Quantitative Immunohistochemical Analysis of Ileum

In situ immunohistochemical analysis of ileum paraffin sections were performed as described previously [28] (link). Primary antibodies against CD3 (#N1580, Dako, Denmark, dilution 1∶10), FOXP-3 (FJK-16s, eBioscience, 1∶100), myeloperoxidase-7 (MPO-7, # A0398, Dako, 1∶500), and F4/80 (#14-4801, clone BM8, eBioscience, 1∶50) were used. For each animal, the average number of positively stained cells within at least six independent high power fields (HPF, 0.287 mm²; 400× magnification) was determined microscopically by two independent double-blinded investigators.

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Heimesaat M.M., Dunay I.R., Schulze S., Fischer A., Grundmann U., Alutis M., Kühl A.A., Tamas A., Toth G., Dunay M.P., Göbel U.B., Reglodi D, & Bereswill S. (2014). Pituitary Adenylate Cyclase-Activating Polypeptide Ameliorates Experimental Acute Ileitis and Extra-Intestinal Sequelae. PLoS ONE, 9(9), e108389.

Publication 2014

N1580 FJK-16s

Animal Antibodies Clone Dilution Ileum Myeloperoxidase Paraffin

Corresponding Organization :

Other organizations : Charité - Universitätsmedizin Berlin, University Hospital Magdeburg, University of Pecs, University of Szeged, Magyar Agrár- és Élettudományi Egyetem

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Variable analysis

independent variables

Primary antibodies used: CD3, FOXP-3, myeloperoxidase-7 (MPO-7), and F4/80

dependent variables

Average number of positively stained cells within at least six independent high power fields (HPF, 0.287 mm^2; 400× magnification)

control variables

Ileum paraffin sections
Immunohistochemical analysis protocol as described previously [28]

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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