Cell Culture Conditions for Burkitt's Lymphoma and Fibroblasts

Ramos human Burkitt’s lymphoma B cells (CRL-1596, American Type Culture Collection, Manassas, VA, USA) were grown in RPMI 1640 medium supplemented with 2 mM L-glutamine and 10% heat-inactivated fetal bovine serum (HyClone, Logan, UT, USA). BALB/3T3 fibroblasts and HEK293 cells were obtained from ATCC (Manassas, VA, USA). HEK293FT human embryonic kidney cells (Thermo Fisher Scientific, San Jose, CA, USA). BALB/3T3 fibroblasts, HEK293 and HEK293FT were maintained in Dulbecco’s modified Eagle’s medium (Sigma, Saint Louis, MO, USA) supplemented with 10% fetal calf serum, 100 μg/mL streptomycin and 100 U/ml penicillin grown in a humidified incubator at 37 °C in 5% CO₂ and 95% air. 3T3/TetOn-AID cells^{10 (link)} were maintained in Dulbecco’s modified Eagle’s medium supplemented with 10% dialyzed fetal calf serum, 100 μg/mL streptomycin and 100 U/ml penicillin grown in a humidified incubator at 37 °C in 5% CO₂ and 95% air.