Quantifying Tear Biomarkers for Clinical Insights

CTSS activity was determined with the CTSS activity kit (BioVision) according to the manufacturer’s instructions. Activity values were normalised to total protein concentration as described previously^{5 (link)}. sIgA, LF, and Cys C levels in tears eluted from Schirmer’s strips were quantified using the sIgA ImmuChrom ELISA Kit (Eagle Biosciences, Amhurst NH), lactoferrin (HLF2) human ELISA Kit (Abcam), and Cys C quantikine human Cys C ELISA kit (R&D Systems), respectively. Final tear dilutions were 1:5,000 for sIgA, 1:60,000 for LF, and 1:50 for Cys C.

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Edman M.C., Janga S.R., Meng Z., Bechtold M., Chen A.F., Kim C., Naman L., Sarma A., Teekappanavar N., Kim A.Y., Madrigal S., Singh S., Ortiz E., Christianakis S., Arkfeld D.G., Mack W.J., Heur M., Stohl W, & Hamm-Alvarez S.F. (2018). Increased Cathepsin S activity associated with decreased protease inhibitory capacity contributes to altered tear proteins in Sjögren’s Syndrome patients. Scientific Reports, 8, 11044.

Publication 2018

CTSS activity kit lactoferrin (HLF2) human ELISA Kit

Ctss Cys cys Dilutions Eagle Elisa Human Lactoferrin Protein Siga Tear

Corresponding Organization :

Other organizations : Southern California Eye Institute, University of Southern California

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

CTSS activity
SIgA levels in tears
LF levels in tears
Cys C levels in tears

control variables

Total protein concentration (used to normalize CTSS activity)

controls

None explicitly mentioned

Annotations

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