Comprehensive Protein Expression Analysis

Western blot procedure was performed according to the method as previously described (14 (link)). The primary antibodies E-cadherin (BD Biosciences, catalog no: 610181 RRID:AB_397581), N-cadherin (BD Biosciences, catalog no: 610920 RRID:AB_398236), Vimentin (BD Biosciences, catalog no: 550513 RRID:AB _393716), iNOS (BD Biosciences, catalog 610332, RRID:AB_397722), β-actin (Sigma-Aldrich, catalog no: MAB1501 clone C4; catalog no: A2066 RRID:AB_476693), Cleaved caspase-9 (Cell Signaling Technology, catalog no: 9502 RRID:AB_2068621); (Cell Signaling Technology, catalog no: 7237 RRID:AB_10895832), Fibronectin [Cell Signaling Technology, catalog no: 26836 Fibronectin/FN1 (E5H6X) Rabbit mAb], Gata4 (Cell Signaling Technology, catalog no: 36966 RRID:AB_2799108), Lamin A/C (Cell Signaling Technology, catalog no: 4777, RRID:AB_10545756), Snail2 (Cell Signaling Technology, catalog no: 9585, RRID:AB_2239535), Twist1 (Cell Signaling Technology, catalog no: 69366, RRID:AB_2891135), Zeb2 [Santa Cruz Biotechnology, catalog no: sc-271984 (E-11)], CD45 (Miltenyi Biotec, catalog no: 130–115–938, RRID:AB_2751284), EpCam (Invitrogen, catalog no: MA5–13917 RRID:AB_11001308); (Novus Biologicals, catalog no: NBP2–27107), CRB2 (ThermoFisher Scientific, catalog no: PA5–25628, RRID:AB_2543128).

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Du Q., Liu S., Dong K., Cui X., Luo J, & Geller D.A. (2022). Downregulation of iNOS/NO Promotes Epithelial–Mesenchymal Transition and Metastasis in Colorectal Cancer. Molecular Cancer Research, 21(2), 102-114.

Publication 2022

E-cadherin N-cadherin Vimentin β-actin Cleaved caspase-9 Fibronectin Gata4 Lamin A/C Snail2 Twist1 EpCam

Actin Antibodies Biologicals Cadherin Caspase 9 Clone Epcam Inos Lamin a c N cadherin Novus Rabbit Twist1 Vimentin Western blot

Corresponding Organization : University of Pittsburgh

Other organizations : First Affiliated Hospital of GuangXi Medical University, Guangxi Medical University, Second Hospital of Anhui Medical University, Anhui Medical University, Second Xiangya Hospital of Central South University, Central South University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Protein levels of E-cadherin, N-cadherin, Vimentin, iNOS, β-actin, Cleaved caspase-9, Fibronectin, Gata4, Lamin A/C, Snail2, Twist1, Zeb2, CD45, EpCam, CRB2

control variables

None explicitly mentioned

controls

The authors do not specify any positive or negative controls for the Western blot procedure.

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