Information was collected by a trained interviewer using a pretested questionnaire and included demographic data, drug history, past medical history of CVD, hypertension, and diabetes and smoking status (20 (link)). After a 15-minute rest in a seated position, using a standardized mercury sphygmomanometer (calibrated by the Iranian institute of standards and industrial researches), two measurements of blood pressure were taken on the right arm. The mean of the two measurements was considered the participant’s blood pressure.
After fasting overnight for 12 to 14 hours, a blood sample was drawn between 7:00 a.m. and 9:00 a.m. from all study participants. All samples were analyzed when internal quality control met the acceptable criteria (19 (link)). All the blood analyses were undertaken on the day of blood collection at the TLGS research laboratory. Total cholesterol (TC) was assayed, using the enzymatic colorimetric method with cholesterol esterase and cholesterol oxidase. High-density lipoprotein cholesterol (HDL-C) was measured after precipitation of the apolipoprotein B containing lipoproteins with phosphotungistic acid. Analyses were performed using Pars Azmon kits (Pars Azmon Inc., Tehran, Iran) and a Selectra 2 auto-analyzer (Vital Scientific, Spankeren, Netherlands). An enzymatic colorimetric method with glucose oxidase was used for the measurement of plasma glucose. The standard two-hour post-challenge plasma glucose (2 hours-PCPG) test was used for participants not on glucose-lowering agents.
After fasting overnight for 12 to 14 hours, a blood sample was drawn between 7:00 a.m. and 9:00 a.m. from all study participants. All samples were analyzed when internal quality control met the acceptable criteria (19 (link)). All the blood analyses were undertaken on the day of blood collection at the TLGS research laboratory. Total cholesterol (TC) was assayed, using the enzymatic colorimetric method with cholesterol esterase and cholesterol oxidase. High-density lipoprotein cholesterol (HDL-C) was measured after precipitation of the apolipoprotein B containing lipoproteins with phosphotungistic acid. Analyses were performed using Pars Azmon kits (Pars Azmon Inc., Tehran, Iran) and a Selectra 2 auto-analyzer (Vital Scientific, Spankeren, Netherlands). An enzymatic colorimetric method with glucose oxidase was used for the measurement of plasma glucose. The standard two-hour post-challenge plasma glucose (2 hours-PCPG) test was used for participants not on glucose-lowering agents.
