Two human ovarian cancer cell lines (A2780, SKOV3), a human cervical adenocarcinoma cell line (Hela) and a human cervical squamous cell carcinoma cell line (Siha) were obtained from the American Type Culture Collection (ATCC, USA).29–32 (link) A2780 and SKOV3 cell lines were maintained in RMPI 1640 medium (Gibco, USA) supplemented with 10% FBS (Biological Industries, Israel). Hela cell lines were cultured in the DMEM medium (Gibco, USA) enriched with 10% FBS. And Siha cell lines were maintained in MEM medium (Gibco, USA) supplemented with 10% FBS. All the cell lines were cultured under the condition of 37°C and 5% CO2.
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Cell Culture of Human Cancer Lines
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Other organizations : Shanghai Tenth People's Hospital, Tongji University
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Variable analysis
independent variables
- Cell lines (A2780, SKOV3, HeLa, SiHa)
- Not explicitly mentioned
- Culture conditions (37°C, 5% CO2)
- Cell culture media (RPMI 1640, DMEM, MEM) supplemented with 10% FBS
- No positive or negative controls were explicitly mentioned in the provided information.
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