Comprehensive Potato Flour Analysis

Total carbon and nitrogen in freeze-dried powderized potato (potato flour) was determined using a Europa 20–20 isotope ratio mass spectrometer with an ANCA preparation system, comprising a combustion and reduction tube operating at 1,000°C and 600°C, respectively. Potato TAG was quantified by chloroform/methanol/water extraction, thin layer chromatography separation, fatty acid methylation and gas chromatography as described in Liu et al. (2017 (link)). Measurements of the total starch and sugar content of potato flour were as described in Liu et al. (2017 (link)). Cellulose was measured using the method of Updegraff (1969 (link)) with an additional digestion of the starch using α-amylase and amyloglucosidase (Total Starch Kit, Megazyme, Wicklow, Ireland). Soluble protein was extracted in buffer (Tris pH 7.5, 10 mM MgCl₂) and quantified by Bradford assay (Bradford, 1976 (link)).

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Mitchell M., Pritchard J., Okada S., Larroque O., Yulia D., Pettolino F., Szydlowski N., Singh S., Liu Q, & Ral J.P. (2017). Oil Accumulation in Transgenic Potato Tubers Alters Starch Quality and Nutritional Profile. Frontiers in Plant Science, 8, 554.

Publication 2017

Total Starch Kit

Amylase Amyloglucosidase Anca Assay Buffer Carbon Cellulose Chloroform Digestion Fatty acid Freeze Gas chromatography Isotope Methanol Methylation Mgcl2 Nitrogen Potato Potato flour Protein Starch Sugar Thin layer chromatography Tris

Corresponding Organization :

Other organizations : Health Sciences and Nutrition, Commonwealth Scientific and Industrial Research Organisation, Miniaturisation pour la Synthèse, l'Analyse et la Protéomique

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Total carbon and nitrogen in freeze-dried powderized potato (potato flour)
Potato triacylglycerol (TAG) content
Total starch and sugar content of potato flour
Cellulose content
Soluble protein content

control variables

None explicitly mentioned

controls

Positive control: None mentioned
Negative control: None mentioned

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