Immunohistochemical Analysis of LMO4 in Breast Tumors

A total of 10 formalin-fixed, paraffin-embedded biopsy breast tumors were available. Immunohistochemistry was performed as described previously (30 (link)). The samples were stained with the LMO4 antibody (dilution, 1:100) used in the western blotting experiments. Diaminobenzidine (DAB; Boster Inc.) was used for color development. The specimens were observed via a Olympus BX51 light microscope (Olympus Corp.) at ×100 magnification.

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Xiong X., Feng Y., Li L., Yao J., Zhou M., Zhao P., Huang F., Zeng L, & Yuan L. (2020). Long non-coding RNA SNHG1 promotes breast cancer progression by regulation of LMO4. Oncology Reports, 43(5), 1503-1515.

Publication 2020

Diaminobenzidine (DAB; BX51 light microscope

Antibody Biopsy Breast tumors Dilution Embedded paraffin Formalin Immunohistochemistry Microscope light

Corresponding Organization :

Other organizations : Second Xiangya Hospital of Central South University, Central South University

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Variable analysis

independent variables

Type of breast tumor (formalin-fixed, paraffin-embedded biopsy tumors)

dependent variables

Expression of LMO4 protein (as measured by immunohistochemistry)

control variables

Antibody dilution (1:100)
Staining method (Diaminobenzidine (DAB) for color development)
Microscope (Olympus BX51 light microscope at ×100 magnification)

controls

No positive or negative controls were explicitly mentioned in the input.

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